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Fig. 3 | Microbial Cell Factories

Fig. 3

From: CRISPR-Cas9 assisted non-homologous end joining genome editing system of Halomonas bluephagenesis for large DNA fragment deletion

Fig. 3

The influence of HR system on gene editing effiency of Mt-NHEJ system in H. bluephagenesis TD01. (A) Growth of H. bluephagenesis TD01 when recA was deleted and overexpressed. (B) Deletion efficiency of phaC gene guided by two sgRNAs when deletion and overexpression of recA. The background expression of recA was used as control. For each experiment of phaC deletion efficiency determination, fifty clones were randomly selected for colony PCR and all experiments were performed in triplicates. The deletion efficiency was calculated by dividing the number of positive deletion clones by the total number of selected clones

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Microbial Cell Factories

ISSN: 1475-2859

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