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Fig. 2 | Microbial Cell Factories

Fig. 2

From: CRISPR-Cas9 assisted non-homologous end joining genome editing system of Halomonas bluephagenesis for large DNA fragment deletion

Fig. 2

Optimization of sgRNAs number and location. (A) Diagrammatic sketch of different position combinations of two and three gRNAs. Blue represents Cas9 protein; green represents PAM sequence; red represents gRNA; Watson is template strand; Crick is coding strand. (B) Deletion efficiency of phaC gene guiding by two and three sgRNAs with different position combinations. For each experiment of phaC deletion efficiency determination, fifty clones were randomly selected for colony PCR and all experiments were performed in triplicates. The deletion efficiency was calculated by dividing the number of positive deletion clones by the total number of selected clones

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Microbial Cell Factories

ISSN: 1475-2859

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