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Fig. 1 | Microbial Cell Factories

Fig. 1

From: CRISPR-Cas9 assisted non-homologous end joining genome editing system of Halomonas bluephagenesis for large DNA fragment deletion

Fig. 1

CRISPR-Cas-NHEJ gene editing system construction and repairing adaptability. (A) Two plasmids system for CRISPR-Cas-NHEJ gene editing system construction. The pCas9-NHEJ (Mt/Ms/Bs) and psgRNA are low copy and high copy plasmid, respectively. The promoter J23119 and Pcas are constitutive promoters. (B) Effect of Mt/Ms/Bs-NHEJ system on the growth of H. bluephagenesis TD01. (C) and (D) The Colony PCR results (agarose gel electrophoresis) of randomly selected clones for phaC deletion under the action of Bs-NHEJ and Mt-NHEJ repair system, respectively. The PCR band of wild type phaC gene was around 1851 bp. The PCR band less than the wild type indicate deletion of the gene segment

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Microbial Cell Factories

ISSN: 1475-2859

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