Table 1 The amino acid contents of WT and gcn4-silenced strains under different nitrogen conditions

Ser

0.43 ± 0.09^bc

0.7 ± 0.17^a

0.55 ± 0.07^ab

0.69 ± 0.07^a

0.44 ± 0.05^bc

0.64 ± 0.1^a

0.37 ± 0.02^c

0.43 ± 0.02^bc

His

2.27 ± 0.71^b

3.22 ± 0.11^a

1.47 ± 0.49^c

2.99 ± 0.51^a

0.51 ± 0.12^d

1.08 ± 0.26 ^cd

0.48 ± 0.02^d

0.59 ± 0.05^d

Gly

1.1 ± 0.06 ^cd

1.35 ± 0.06^c

1.11 ± 0.19 ^cd

2.64 ± 0.08^a

2.08 ± 0.39^b

0.88 ± 0.14^d

2.18 ± 0.22^b

0.85 ± 0.1^d

Thr

2.23 ± 0.13^a

1.27 ± 0.09^c

1.78 ± 0.05^b

0.95 ± 0.06^d

1.14 ± 0.21 ^cd

1.27 ± 0.28^c

0.42 ± 0.17^e

0.98 ± 0.09^d

Arg

2.6 ± 0.34^a

2.68 ± 0.32^a

2.97 ± 0.35^a

2.69 ± 0.09^a

1.1 ± 0.12^d

2.06 ± 0.11^b

0.82 ± 0.06^d

1.61 ± 0.1^c

Ala

2.82 ± 0.48^b

3.62 ± 0.24^a

2.52 ± 0.11^b

3.45 ± 0.23^a

2.01 ± 0.29^c

1.26 ± 0.14^d

1.29 ± 0.23^d

0.67 ± 0.04^e

Tyr

2.42 ± 0.28^d

3.09 ± 0.46^bc

2.54 ± 0.29 ^cd

3.18 ± 0.25^b

1.76 ± 0.53^e

4.15 ± 0.11^a

1.3 ± 0.24^e

3.44 ± 0.19^b

Cys

2.41 ± 0.31^ab

2.55 ± 0.37^ab

2.66 ± 0.42^ab

3.06 ± 1.32^a

1.64 ± 0.16^bc

1.83 ± 0.21^bc

1.33 ± 0.28^c

1.95 ± 0.23^bc

Val

3.05 ± 0.75^c

5.41 ± 0.63^a

2.54 ± 0.09^c

5.05 ± 0.84^ab

0.73 ± 0.19^d

4.32 ± 0.31^b

0.92 ± 0.1^d

4.52 ± 0.39^ab

Met

0.25 ± 0.02^c

1.03 ± 0.08^a

0.65 ± 0.37^b

1.23 ± 0.16^a

0.53 ± 0.1^b

1.19 ± 0.2^a

0.62 ± 0.12^b

1.04 ± 0.1^a

Phe

0.92 ± 0.18^d

2.38 ± 0.02^a

1.08 ± 0.26^d

2.51 ± 0.34^a

0.44 ± 0.02^e

1.54 ± 0.17^c

0.47 ± 0.01^e

1.9 ± 0.21^b

Ile

0.5 ± 0.17^b

2.4 ± 0.11^a

0.64 ± 0.2^b

2.65 ± 0.34^a

0.6 ± 0.03^b

0.66 ± 0.19^b

0.5 ± 0.12^b

0.69 ± 0.03^b

Leu

3.92 ± 0.69^b

7.39 ± 0.21^a

2.79 ± 0.18^bc

6.29 ± 0.44^a

1.36 ± 0.37^c

0.68 ± 0.12^c

1.04 ± 0.32^c

5.58 ± 0.1^b

Lys

2.87 ± 0.47^d

9.41 ± 0.24^a

3.61 ± 0.12^c

8.46 ± 0.86^b

0.41 ± 0.18^f

10.08 ± 0.31^a

1.59 ± 0.18^e

8.07 ± 0.12^b

Trp

0.68 ± 0.31^d

1.66 ± 0.03^a

0.57 ± 0.1^d

1.35 ± 0.27^ab

0.54 ± 0.08^d

1.54 ± 0.47^ab

0.83 ± 0.07 ^cd

1.16 ± 0.17^bc