Skip to main content
Fig. 4 | Microbial Cell Factories

Fig. 4

From: The transcription factor GCN4 contributes to maintaining intracellular amino acid contents under nitrogen-limiting conditions in the mushroom Ganoderma lucidum

Fig. 4

GCN4 inhibits TORC1 activity under low-nitrogen conditions. Western blot analysis of S6K phosphorylation levels in the WT and gcn4-silenced strains cultured under 3 mM and 60 mM Asn, with histograms showing the relative ratio of p-S6K/Total-S6K. The p-S6K/Total-S6K ratio in the WT strain grown under 60 mM Asn was defined as 1. The data are presented as the mean ± SD (n = 3). Statistical significance is represented by different letters according to Tukey′s test (P < 0.05)

Back to article page