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Fig. 3 | Microbial Cell Factories

Fig. 3

From: The transcription factor GCN4 contributes to maintaining intracellular amino acid contents under nitrogen-limiting conditions in the mushroom Ganoderma lucidum

Fig. 3

GCN4 is involved in regulating the TCA cycle and glycolysis under low-nitrogen conditions

(A) Relative activities of key enzymes (ICDH and KGDH) associated with the TCA cycle in the WT and gcn4-silenced strains under 3 mM and 60 mM Asn (B) Relative expression of key enzyme synthesis genes in the TCA cycle of the WT and gcn4-silenced strains under 3 mM and 60 mM Asn. (C) Relative activities of key enzymes (PFK, HK and PK) associated with glycolysis in the WT and gcn4-silenced strains under 3 mM and 60 mM Asn. (D) Relative expression of key enzyme synthesis genes in glycolysis in the WT and gcn4-silenced strains under 3 mM and 60 mM Asn. hk: hexokinase. pfk: 6-phosphofructokinase. pk: pyruvate kinase. cs: citrate synthase. icdh: isocitrate dehydrogenase. kgdh: α-ketoglutarate dehydrogenase. The data are presented as the mean ± SD (n = 3). Statistical significance is represented by different letters according to Tukey′s test (P < 0.05)

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Microbial Cell Factories

ISSN: 1475-2859

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