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Fig. 2 | Microbial Cell Factories

Fig. 2

From: The transcription factor GCN4 contributes to maintaining intracellular amino acid contents under nitrogen-limiting conditions in the mushroom Ganoderma lucidum

Fig. 2

Involvement of GCN4 in regulating intracellular amino acid biosynthesis-related genes under low-nitrogen conditions. Relative expression of genes related to amino acid synthesis and transport in WT and gcn4-silenced strains under 3 mM and 60 mM Asn conditions. Expression of the asparagine synthetase gene (asns), glutamine synthetase gene (gs), glutamate synthase gene (gogat), glutamic oxaloacetic transaminase genes (got1 and got2) and glutamic-pyruvic transaminase gene (gpt), as well as 7 that of amino acid transport genes (Gl23068, Gl29937, Gl20736, Gl21744, Gl23271, Gl28933, Gl23088), were determined. Expression levels of genes in the WT strain cultured under 60 mM Asn were defined as 1. The data are presented as the mean ± SD (n = 3). Statistical significance is represented by different letters corresponding to P < 0.05 based on Tukey′s test

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Microbial Cell Factories

ISSN: 1475-2859

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