Fig. 1

Involvement of GCN4 in regulating intracellular amino acid contents. Intracellular amino acid contents (A), essential amino acid contents (B), MSG-like flavour amino acid contents (C), sweet flavour amino acid contents (D), bitter flavour amino acid contents (E) and flavourless amino acid contents (F) in WT and gcn4-silenced strains. MSG-like flavour amino acids include aspartic acid and glutamic acid. Sweet flavour amino acids include threonine, serine, glycine and alanine. Bitter flavour amino acids include valine, methionine, isoleucine, leucine, phenylalanine, histidine, arginine and tryptophan. Flavourless amino acids include cystine, tyrosine and lysine. The data are presented as the mean ± SD (n = 3). Statistical significance is represented by different letters corresponding to P < 0.05 based on Tukey′s test