Table 1 Strains and plasmids used in this study
Strains/plasmids | Genotype | Sources |
|---|---|---|
DH5 alpha | dlacZ Delta M15 Delta(lacZYA-argF) U169 recA1 endA1 hsdR17(rK-mK+) supE44 thi-1 gyrA96 relA1 | Lab Stock |
Lemo21(DE3) | fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS/pLemo(CamR) λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5 pLemo = pACYC184-PrhaBAD-lysY | New England Biolabs |
BL21(DE3) | F – ompT hsdSB (rB- mB-) gal dcm (DE3) | Lab Stock |
pUC57a | Ampr, cloning vector | Genwiz |
pET32a | Ampr, expression vector | Lab Stock |
pSB2K3 | Kanr, IPTG-inducible vector | iGEM Foundation |
pSB4K5 | Kanr, expression vector | iGEM Foundation |