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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Improved production of β-carotene in light-powered Escherichia coli by co-expression of Gloeobacter rhodopsin expression

Fig. 5

a SDS-PAGE of RFP in E. coli BL21 (DE3) expressing GR and its controls. (M: blue-elf pre-stained marker, RFP: lane 1, RFP_pET32a: Lane 2, RFP_GR: Lane 3, each well loaded with 16 µg E. coli total protein). b RFP intensity prior to the protein assay in GR-host and its wildtype control (RFP) and vector control (RFP_pET32a) (****: p-value < 0.0001, done with t-test, triplicate). c Growth pattern of RFP intensity in LB batch in GR-GFP host with its vector control (pET32a). Overall, GR-host presented a significant improved of the expression of RFP in its total protein yield and also reflected on its fluorescence intensity during the growth

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Microbial Cell Factories

ISSN: 1475-2859

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