Fig. 7
Multiomics view of the DHA-producing Y. lipolytica strain genealogy. The absolute levels of intracellular CoA thioesters were determined using LC‒MS/MS and internal 13C standards (A). Samples were taken during the early production phase (20 h after the maximum biomass concentration had been reached). The data were used to inspect the relation between the intracellular levels of acetyl-CoA and malonyl-CoA, the two precursors for DHA biosynthesis (B), and the intracellular levels of malonyl-CoA and DHA production (C). In addition, gene expression data in the different producers are shown in relation to DHA production (D, E). Here, the total expression strength, i.e., the sum of the average expression of all four cluster genes during the DHA production phase (D), as well as the expression ratio between pfa2 + pfa3 versus pfa1 + ppt (E) during the DHA production phase, are given. The values represent the average expression after 38 and 135 h. n = 3