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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Refactoring the architecture of a polyketide gene cluster enhances docosahexaenoic acid production in Yarrowia lipolytica through improved expression and genetic stability

Fig. 5

Evaluation of the genetic stability of heterologous PUFA clusters expressed in recombinant Y. lipolytica to produce docosahexaenoic acid (DHA). The different genetic cluster layouts can be taken from Fig. 3. The presence of the four cluster genes, pfa1, pfa2, pfa3, and ppt, was analysed by colony PCR (A). The use of four specific primer pairs resulted in PCR products with clearly distinguishable lengths: 1.72 kb (pfa1), 0.91 kb (pfa2), 0.73 kb (pfa3), and 0.61 kb (ppt), enabling simultaneous analysis. For each strain, we analysed the genetic configuration of 40 clones from cell populations sampled from the production process after 12 h (B, early phase) and 185 h (C, late phase). The DHA production efficiency for the two phases was estimated after 40 h (early phase) and 185 h (late phase). The relative DHA production is given in comparison to the best strain during each phase, set to 100%. n = 3

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Microbial Cell Factories

ISSN: 1475-2859

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