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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Refactoring the architecture of a polyketide gene cluster enhances docosahexaenoic acid production in Yarrowia lipolytica through improved expression and genetic stability

Fig. 3

Metabolic engineering of Yarrowia lipolytica to produce docosahexaenoic acid (DHA) via differently designed heterologous PUFA gene clusters. The cluster designs were based on different combinations of genetic control elements to express each of the four cluster genes (pfa1, pfa2, pfa3, ppt), including UAS1B element blocks of different lengths, the two core promoters pTEF and pminLEU2, the 5ʹ TEF intron, 200 bp spacers, and the Lip2t terminator (A). The created strains were evaluated for production efficiency in glycerol-grown batch cultures, whereby the DHA level (mg gCDM−1) was measured after 185 h (B, C, D). The data show the genetic layout and performance of first-generation strains based on the TEF promoter (B) and second-generation strains based on the minLEU2 promoter (C, D). n = 3

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Microbial Cell Factories

ISSN: 1475-2859

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