Fig. 3
From: Cytotoxic effects of halophilic archaea metabolites on ovarian cancer cell lines
Real time impedance in A2780 cells treated with archaeal samples monitored using xCELLigence system. A2780 cells at 3 × 104 cells/well were incubated for 24 h at 37 °C with 5% CO2 in an electronic 96-well plate and then treated with 10, 100 or 200 µg/mL of Nnm. salaciae (NS, upper panel), Hrd. rudnickae 64T (HR64, middle panel) or Hrd. rudnickae 66 (HR66, lower panel) extract, with fresh culture medium extract or were left untreated, as indicated, for the following 72 h. During incubation, impedance was measured every 15 min in each individual well and automatically converted to Cell Index (CI) values. The graph presents characteristic curves of cells’ behaviour before and during treatment