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Table 3 Overview of the different plasmids used in this study

From: Engineering Comamonas testosteroni for the production of 2-pyrone-4,6-dicarboxylic acid as a promising building block

Plasmid ID

Plasmid content

Reference

pBBR1MCS-2

Host plasmid with Kmr and pBBR1 ori

[33]

pCT01

Suicide plasmid with Tetr and pUC ori

This study

pCT02

pCT01, pmdD deletion vector

This study

pCT03

Host plasmid with Chlorr and pBBR1 ori

This study

pCT04

pCT03 with PTPA, I-SceI

This study

pCT05

pCT04 with pmdD recovery cassette

This study

pCT06

pCT04 with pmdD and pmdK recovery cassette

This study

pCT07

pCT03 with PTPA, mCherry

This study

pCT08

pCT03 with PPCA, mCherry

This study