Fig. 3From: A fine-tuned yeast surface-display/secretion platform enables the rapid discovery of neutralizing antibodies against Clostridioides difficile toxinsSecretion of Fab-AH3/E3 at different conditions. Y-AH3/E3 cells were induced to produce Fab in synthetic minimal media containing various carbon sources for (a) 48Â h or (b) 72Â h as the indicated concentration (w/v). The supernatants were collected, and western blot was performed to detect Fab with anti-HA tag antibodyBack to article page