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Fig. 1 | Microbial Cell Factories

Fig. 1

From: A fine-tuned yeast surface-display/secretion platform enables the rapid discovery of neutralizing antibodies against Clostridioides difficile toxins

Fig. 1

Schematic representation of Fab display or secretion platform. (a) The encoding gene cassettes of Aga1, variable region-HC-Aga2 and variable region-LC. The gene encoding Aga1 is located on a chromosome. The genes encoding HC and LC are located on two separate plasmids. (b) Fab display and secretion. Diploid yeast harboring both plasmids encoding HC and LC will display Fab on cell wall via Aga1-Aga2 interaction when induced by galactose but will secrete Fab into the culture supernatant when a carbon source other than galactose is present

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Microbial Cell Factories

ISSN: 1475-2859

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