Fig. 5
Screening of a SP-Cut11 library. A The cut11 gene was fused to B. subtilis signal peptide library. The secretion biosensor B. subtilis PAL5 was transformed with the SP library and 480 clones were analyzed in vivo regarding the enzymatic activity using the substrate pNPP (grey, activity), the protein amount as determined by split GFP fluorescence (green, split GFP fluorescence) and the corresponding secretion stress as indicated by mCherry fluorescence (red, mCherry fluorescence) The construct 4nt-SPPel-cutinase was taken as benchmark (dotted line) B Selected variants were transferred to B. subtilis PAL5 and the resulting strains were cultivated, analyzed, and the corresponding signal peptides were identified by DNA sequencing