Fig. 3
Schematic illustration of the cutinase expression plasmids. The expression plasmids harbor a Shine Dalgarno sequence (SD, yellow), a spacer region (brown), the start codon of the target gene (red line), the respective signal sequence (SPPel [dark green], SPEpr [blue] or SPBsn [violet]), cut (orange) and the gfp11 tag (light green). The nucleotide sequences covered by the Shine Dalgarno (SD) sequence and the spacer region with lengths varying from 4 to 12 nucleotides (nt) by insertion of adenosines are shown