Economical production of Pichia pastoris single cell protein from methanol at industrial pilot scale

Table 2 Changes in total nitrogen and protein content of different P. pastoris strains in 0.5% methanol minimal medium

Strains	Total nitrogen (g/g DCW)	Protein content (g/g DCW)	Increased % ratio compared with X-33 at 30 °C
X-33 (30 °C)	0.058 ± 0.004	0.364 ± 0.028	0
X-33 (33 °C)	0.064 ± 0.002^*	0.400 ± 0.012^*	10%
HTX-33	0.059 ± 0.002	0.368 ± 0.016	1%
HTX-33-GDH1	0.063 ± 0.003^*	0.394 ± 0.016^*	8%
HTX-33-GLN1	0.066 ± 0.003^*	0.414 ± 0.009^*	14%
HTX-33-GDH1-GLN1	0.065 ± 0.001^*	0.413 ± 0.007^*	13%
HTX-33-ΔPAS_chr4_0305	0.071 ± 0.002^**	0.444 ± 0.012^**	22%
HTX-33-GLN1-ΔPAS_chr4_0305	0.073 ± 0.004^***	0.456 ± 0.025^***	25%

Unless otherwise specified, P. pastoris strains were cultivated in 48-well plates containing 0.5% methanol minimal medium at 33 °C at 800 rpm. The total nitrogen and protein content of P. pastoris strains were determined when grown to the exponential phase. Data are average values and standard deviations of triplicate experiments. An asterisk indicates a significant difference with ^*P < 0.05, ^**P < 0.01, ^***P < 0.001

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