Table 1 Strains and plasmids used in this study
From: Economical production of Pichia pastoris single cell protein from methanol at industrial pilot scale
Strains and Plasmids | Relevant Characteristics | Sources |
|---|---|---|
P. pastoris | Â | Â |
X-33 | Wild type, Mut+ | Lab stock |
HTX-33 | Derived from X-33 through ALE | This study |
HTX-33-GDH1 | Derived from HTX-33, PNSII-3::PAOX1-GDH1-TADH2 | This study |
HTX-33-GLN1 | Derived from HTX-33, PNSI-2::PDAS2-GLN1-TADH2 | This study |
HTX-33-GDH1-GLN1 | Derived from HTX-33, PNSII-3::PAOX1-GDH1-TADH2; PNSI-2::PDAS2-GLN1-TADH2 | This study |
HTX-33-ΔPAS_chr4_0305 | Derived from HTX-33, ΔPAS_chr4_0305 | This study |
HTX-33-GLN1-ΔPAS_chr4_0305 | Derived from HTX-33, PNSI-2:: PDAS2-GLN1-TADH2; ΔPAS_chr4_0305 | This study |
E. coli | Â | Â |
DH5a | F−, φ80lacZ ΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk−, mk+), phoA, supE44, λ−, thi-1, gyrA96, relA1 | Lab stock |
Plasmids | Â | Â |
pPICZ-Cas9-gGUT1 | ori, Amp, Zeocin, TDAS1-Cas9-PHTX1-GUT1-gRNA2-TAOX | (22) |
pPICZ-Cas9-gPNSI-2 | ori, Amp, Zeocin, TDAS1-Cas9-PHTX1- PNSI-2-gRNA-TAOX | This study |
pPICZ-Cas9-gPNSII-3 | ori, Amp, Zeocin, TDAS1-Cas9-PHTX1- PNSII-3-gRNA-TAOX | This study |
pPICZ-Cas9-gPAS_chr4_0305 | ori, Amp, Zeocin, TDAS1-Cas9-PHTX1-PAS_chr4_0305-gRNA-TAOX | This study |