Table 1 Strains and plasmids used in this study
From: Synthesis of isobutanol using acetate as sole carbon source in Escherichia coli
Strains and Relevant characteristic plasmids | Source | |
|---|---|---|
Strains | Â | |
BW25113 | F−, λ−, rph-1 | Lab stock |
DH5α | F−, endA1, hsdR17 (rK−, mK+), supE44, thi-l, l−, recA1, gyrA96, ΔlacU169 (Φ80lacZ ΔM15) | Lab stock |
BWΔPPAL | BW25113 (ΔpflBΔpoxBΔadhEΔldhA) | This study |
NH000 | BWΔPPAL containing pTrc99a-1 and pCL1920-1 | This study |
NH001 | BWΔPPAL containing pTrc99a-2 and pCL1920-1 | This study |
NH002 | BWΔPPAL containing pTrc99a-2 and pCL1920-2 | This study |
WY001 | BWΔPPAL containing pTrc99a-3 and pCL1920-1 | This study |
WY002 | BWΔPPAL containing pTrc99a-3 and pCL1920-2 | This study |
Plasmids | Â | Â |
pKD3 | oriR6Kγ, bla(AmpR), cat, rgnB(Ter) | [46] |
pKD4 | oriR6Kγ, bla(AmpR), kan, rgnB(Ter) | [46] |
pTKRed | SpcR | [47] |
pCP20 | bla and cat, helper plasmid | [48] |
pTrc99a | AmpR | Lab stock |
pTrc99a-1 | pTrc99a-adhA-kivD-alsS | This study |
pTrc99a-2 | pTrc99a-adhA-kivD-alsS-ydbK | This study |
pTrc99a-3 | pTrc99a-adhA-kivD-alsS-pckA-maeB | This study |
pCL1920 | SpcR | Lab stock |
pCL1920-1 | pCL1920-alsS-ilvC-ilvD-acs | This study |
pCL1920-2 | pCL1920-alsS-ilvC-ilvD-acs-pntAB-yfjB | This study |