Table 1 Strains and plasmids used in this study
From: Analysis of acid-tolerance mechanism based on membrane microdomains in Saccharomyces cerevisiae
| Â | Description | Source |
|---|---|---|
Strains | ||
 E. coli JM109 | E. coli, for plasmid construction | Lab stock |
 WT | CEN.PK2-1C, MATa; his3D1; leu2-3_112; ura3-52; trp1-289; MAL2-8c; SUC2 | Lab stock |
 TAMC | CEN.PK2-1C derivate, which can tolerate a low pH of 2.3 | [15] |
 WT-D4 | WT derivate, expressing the plasmid pY15-GFP-D4 | This study |
 TAMC-D4 | TAMC derivate, expressing the plasmid pY15-GFP-D4 | This study |
 TAMC-E | TAMC derivate, ΔERG6 | This study |
 WT-E | WT derivate, ΔERG6 | This study |
 WT-PP | WT derivate, expressing the plasmid pY15-PMP1-mCherry-PMA1-EGFP | This study |
 TAMC-PP | TAMC derivate, expressing the plasmid pY15-PMP1-mCherry-PMA1-EGFP | This study |
Plasmids | ||
 pY15 | Amp, LEU2, CEN/ARS, E. coli-S. cerevisiae shuttle vector | Lab stock |
 pY15-GFP-D4 | pY15 derivate, inserting PTEF1-GFP-D4-TCYC1 | This study |
 pY15-PMP1-mCherry-PMA1-EGFP | pY15 derivate, inserting PTEF1-PMP1-mCherry-TCYC1 and PGAP-PMA1-EGFP-TAOX1 | This study |