Fig. 6

Colocalization of PMA1–GFP and PMP1–mCherry in WT, TAMC, and TAMC-E strains in different YPD media. A Colocalization analysis of PMA1–EGFP and PMP1–mCherry in WT, TAMC, and TAMC-E strains at pH 6.0. B Colocalization analysis of PMA1–EGFP and PMP1–mCherry in WT, TAMC, and TAMC-E strains at pH 2.3. The first three columns indicate the confocal dual-channel imaging and merging images of PMA1–EGFP and PMP1–mCherry. The fourth column is the plot of pixel intensity along the white line from left to right of each plot, with colors in merged images. The fifth column represents the scattergram of the first and second lines. Scale bar, 5 µm