Fig. 3

Analysis of membrane order degree in WT and TAMC strains. A Confocal dual-channel imaging of WT and TAMC strains labeled using di-4-ANEPPDHQ. The first and second left images indicate green (500–580 nm) and red (620–750 nm) channel images, respectively; the third middle image represents the superposition of red and green channels; the second right image is the HSB image; and the first right image is the surface plot of the ratio. The first and second lines are the confocal dual-channel imaging of TAMC and WT strains in YPD medium at pH 6.0, respectively. The third and fourth lines are the confocal dual-channel imaging of TAMC and WT strains in YPD medium at pH 2.3. Scale bar, 5 μm. B Histogram of GP value distribution of the membranes of WT and TAMC strains in YPD medium at pH 6.0 and 2.3. C GP value of the membranes of WT and TAMC strains in YPD medium at pH 6.0 and 2.3. Scale bar, 5 µm