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Fig. 6 | Microbial Cell Factories

Fig. 6

From: A temperature dependent pilin promoter for production of thermostable enzymes in Thermus thermophilus

Fig. 6

Purification of MtlD from T. kivui produced in T. thermophilus. The enzyme was purified by Ni-NTA (elution with 300 mM imidazole) and analyzed on a 12% SDS gel. The protein was stained with Coomassie brilliant blue R-250 (A). Western blot analysis of the fractions obtained during purification of MtlD-his. Western blot analysis was performed using the penta-his antibody (1:1000 dilution) (B). The white arrow indicates MtlD-his.

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Microbial Cell Factories

ISSN: 1475-2859

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