Table 3 Strains and plasmid used in the study
Name | Description | References |
|---|---|---|
E. coli DH5α | Host strain for cloning |  |
Saccharomyces cerevisiae NGY10 | Ethanol production | [29] |
Candida tropicalis K2 | Parental strain | [15] |
Candida tropicalis K2M | EMS mutated C. tropicalis K2 | This study |
Candida tropicalis K21D | Single allele deleted C. tropicalis K2 (XYL2/xyl2Δ::FRT) | This study |
Plasmids | ||
 pSFS2A | Plasmid containing FRT-MAL2p-FLP-SAT1-FRTcassette | [49] |
 pSFS2A-XYL2-I | pSFS2A containing 5′XYL2-FRT-SAT1-FLP-FRT-3′XYL2 disruption cassette | This study |