Table 1 Statistical screening of factors influencing the biosynthesis of bioemulsifier by S. cerevisiae strain MYN04 via the PB
Run # | KH2PO4 | MgSO4 | Yeast Extract | Sucrose | pH | Inoculum Size | Volume | CaCl2 | FeSO4 | NaCl | EI24, % |
|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 1.5 | 2.5 | 7 | 25 | 3.0 | 3 | 125 | 0.3 | 0.01 | 0.1 | 36.5 |
2 | 0.5 | 7.5 | 3 | 25 | 7.0 | 1 | 125 | 0.3 | 0.04 | 0.1 | 64.6 |
3 | 1.5 | 7.5 | 7 | 15 | 3.0 | 1 | 125 | 0.1 | 0.04 | 1.0 | 32.7 |
4 | 0.5 | 7.5 | 7 | 25 | 3.0 | 1 | 75 | 0.3 | 0.01 | 1.0 | 43.1 |
5 | 1.5 | 2.5 | 3 | 15 | 7.0 | 1 | 125 | 0.3 | 0.01 | 1.0 | 57.7 |
6 | 0.5 | 2.5 | 3 | 15 | 3.0 | 1 | 75 | 0.1 | 0.01 | 0.1 | 44.0 |
7 | 1.5 | 2.5 | 7 | 25 | 7.0 | 1 | 75 | 0.1 | 0.04 | 0.1 | 62.0 |
8 | 1.0 | 5.0 | 5 | 20 | 5.0 | 2 | 100 | 0.2 | 0.025 | 0.55 | 52.8 |
9 | 1.0 | 5.0 | 5 | 20 | 5.0 | 2 | 100 | 0.2 | 0.025 | 0.55 | 52.8 |
10 | 1.5 | 7.5 | 3 | 25 | 7.0 | 3 | 75 | 0.1 | 0.01 | 1.0 | 43.1 |
11 | 0.5 | 2.5 | 7 | 15 | 7.0 | 3 | 75 | 0.3 | 0.04 | 1.0 | 58.5 |
12 | 1.5 | 7.5 | 3 | 15 | 3.0 | 3 | 75 | 0.3 | 0.04 | 0.1 | 55.6 |
13 | 0.5 | 2.5 | 3 | 25 | 3.0 | 3 | 125 | 0.1 | 0.04 | 1.0 | 48.1 |
14 | 1.0 | 5.0 | 5 | 20 | 5.0 | 2 | 100 | 0.2 | 0.025 | 0.55 | 56.6 |
15 | 0.5 | 7.5 | 7 | 15 | 7.0 | 3 | 125 | 0.1 | 0.01 | 0.1 | 57.7 |