Fig. 2
From: Metabolic engineering of fast-growing Vibrio natriegens for efficient pyruvate production
Improvement of pyruvate production by pathway engineering. A Schematic diagram of metabolic engineering strategies. The genes marked in red color are essential genes for the biosynthesis of byproducts. B Growth assay of PYR02 and other engineered strains. C Pyruvate titers of PYR02 and other engineered strains. D Pyruvate titers of per OD600 cells for PYR02 and other engineered strains. E Acetate titers of PYR02 and other engineered strains. F qRT-PCR analysis of aceE transcription level. G qRT-PCR analysis of ppc transcription level. The experiments were performed in shake flasks and incubated at 37 ℃ and 250 rpm for 24 h. Three independent replicates were performed. Data with error bars represent the means and standard deviations