Table 1 Comparison of several commercial mini-bioreactor systems and their applications in modern bioprocess development/research
From: High-throughput process development from gene cloning to protein production
Name | BioLector | Micro-24 | Micro-matrix | 2mag bioreactor 48 | Ambr15f |
|---|---|---|---|---|---|
Characteristics | Microplate-based bioreactor; Operates with non-invasive optical sensors; Real time culture monitoring; Can be upgraded to fully automated units | Bubble column-based bioreactor; 24 simultaneous cultures with independent control of each reactor; individual pH, DO, and temperature sensors ; Real time monitoring | Microplate-based mini-bioreactor; 24 simultaneous cultures with independent control of each reactor; Individual liquid addition; Real time monitoring | Microplate-based mini-bioreactor; 48 parallelized mini reactors; Non-invasive real-time measurement of pH and DO | Micro-stirred tank bioreactor; Single-use pH and DO sensors; Available with temperature compensation for low-temperature application; Real time monitoring |
Working volume | 0.8–1.5 mL/well | 3–7 mL/column | 1–7 mL/well | 8–15 mL/well | 10–15 mL/vessel |
Availability of feed | Require an external liquid handling station | Integrated liquid feed individually per column | Integrated liquid feed individually per bioreactor | Require the integration of a pipetting robot | Integrated liquid feed individually per vessel |
Application areas | Cell and strain screening; Growth characterization analysis; Medium screening and optimization; Culture condition optimization; Systematic biology; Gene/proteomics studies | Clone screening; Optimizing temperature, pH, and DO conditions for cell growth; Optimizing the induction condition for protein expression; Optimizing medium composition; Process development studies | Screening cell-line, microbial, and/or yeast libraries; Process development studies; Process optimization studies; Small volume cultivations | Clone selection; Media screening and optimization; Optimization of the process design; Studies of gene and protein expression | Clone selection; Media and feed optimization; Process intensification; Development of advanced cell therapies |
Reference | |||||
Website of suppliers | https://www.2mag.de/en/produkte-e/bioreactor-e/bioreactor-48-e.html |