Fig. 3
From: High-throughput process development from gene cloning to protein production
Schematic diagram and the principle of the SLiCE method and LIC method. (A) Overview of SLiCE cloning. Target genes are flanked by 15–19 bp recombination sites. Laboratory E. coli strains´ SLiCE-mediated recombination between the homologous arms generates the final vector. (B) A schematic for the production of recombinant DNA through LIC cloning. The linearized expression vector and target gene containing complementary tails are digested by T4 DNA polymerase (3’ exo) and then transformed into E. coli for in vivo ligation after annealing