Fig. 6

Pinpointing minimal Hsp150 region needed for efficient N-terminally anchored surface display. (A) Summary tracks describing construct v5 and deletions introduced into constructs Δ1, Δ2, Δ3, Δ4, Δ5, Δ6 and Δ7, with deleted regions marked as black lines, and the visualisations of Alphafold2-structural models comparing constructs v5, Δ1, and Δ5. (B) Relative β-lactamase activity of fusion constructs v5, Δ1, Δ2, Δ3, Δ4, Δ5, Δ6 and Δ7, normalised to that of construct v5, with error bars denoting standard deviations and bar colours corresponding to those in panel A. (C) Anti-HA immunoblotting of the noncovalently- and covalently-bound cell wall proteins