Fig. 5

Shuffling the β-lactamase within the Hsp150-fusion protein affects surface display. (A) Alphafold2-structural model of Hsp150 protein fused with the tag-rich disordered linker. Black triangles point to the β-lactamase insertion sites. The colour scheme of the structural model and the summary tracks follow Fig. 1. (B) Relative β-lactamase activity of fusion constructs v1, v2, v3, v4, and v5, normalised to that of construct v5, with error bars denoting standard deviations and bar colours indicating the position of the inserted β-lactamase within the structural model. (C) Anti-HA immunoblotting of the noncovalently- and covalently-bound cell wall proteins