Fig. 4

Saturating secretory pathway with Hsp150-ymScarletI fusion protein does not affect the cell wall. (A) Alphafold2-structural model of the Hsp150-ymScarletI fusion protein. The colour scheme of the structural model and the summary tracks follows Fig. 1. (B) Confocal micrograph of the wild-type cells stained with FITC-conjugated concanavalin A. Scale bar denotes 5.0 μm. (C) Confocal micrograph of Hsp150-ymScarletI-expressing cells stained with FITC-conjugated concanavalin A. (D) Cell size and the amounts of mannoprotein, chitin, and β-glucan of wild-type (white) and Hsp150-ymScarletI-expressing cells (red), quantified via flow cytometry and FITC-conjugated concanavalin A-, calcofluor white-, and aniline blue-staining, respectively. Graphs show two biological replicates