Fig. 3

 S. cerevisiae lacks full-length Pir6, instead encoding 50 aa-long Ykl162c-a. (A) An alignment of Ykl162c-a and Pir6 homologues, with red letters indicating mismatches and light orange boxes highlighting signal sequences. The lower inset shows the alignment between S. cerevisiae and S. paradoxus loci encoding Ykl162c-a, i.e., Pir6 C-terminus, with orange letters indicating mismatches. (B) Immunoblot of the C-terminally Myc-tagged Ykl162c-a, expressed from its native promoter (left) and from constitutive strong TEF1 promoter (right), analysed in biological triplicates from exponentially growing cultures. (C) Heatmaps visualising gene expression from the Watson (upper heatmaps) and Crick strands (lower heatmaps) of diploid S. cerevisiae YKL162c-A locus show that the locus is expressed in the respiring (YPA) and sporulating (1-12 h in SP medium) but not in the fermenting diploid cells (YPD). The colour scale shifts from blue (not expressed) through white to red (strongly expressed)