Fig. 4

A3D predictions for yeast hexokinase GLK1 self-assembly. (a) Cryo-EM structure of the yeast hexokinase GLK1 filament (PDB: 6PDT). (b) Structure of a GLK1 monomer showing the location of the N-terminal Phenylalanine residue involved in filament formation (Phe3, indicated with a circle). (c) A3D prediction of the wild-type active monomeric GLK1 obtained from the A3DyDB. Predicted aggregation propensities are colored in the structure ranging from blue (more solubilizing) to red (more aggregation-prone). The position of the two predicted hydrophobic STAPs is indicated with arrows. (d) Detailed representation of the insertion site of Phe3 into the C-terminus hydrophobic cavity. (e) A3D prediction of the Phe3Ser (P3S) solubilizing mutant. This solubilizing mutation eliminates filament polymerization