Fig. 2

Validation of long-term rbALE workflow. (A) Example dataset rbALE Glc R1 out of six independent evolution experiments using the MPP technology. C. glutamicum WT was cultivated for a total number of 16 repetitive batches in one FlowerPlate and defined CGXII medium with 111 mM glucose as sole carbon and energy source. The resulting online BS measurements (meas) were used for process model fitting (fit). (B) Estimated specific growth rates across the single batches of all six rbALE runs