Skip to main content

Table 1 Corynebacterium glutamicum strains used in this study

From: A 4-hydroxybenzoate 3-hydroxylase mutant enables 4-amino-3-hydroxybenzoic acid production from glucose in Corynebacterium glutamicum

Strain

Relevant characteristics

Reference

NBRC 12168

Wild type C. glutamicum strain identical to ATCC 13032

aNBRC

NBRC 12169

Wild type C. glutamicum strain identical to ATCC 13058

NBRC

HT23

NBRC 12168 derivate; ΔcglIM (cg1996), ΔcglIR (cg1997), ΔcglIIR (cg1998)

[62]

KC265

HT23 derivate; Ptuf_aroG (cg2391)

This study

KC282

KC265 derivate; Ptuf_aroE3 (cg1835)

This study

KC300

KC282 derivate; Ptuf_aroB (cg1827)

This study

KC314

KC300 derivate; Ptuf_aroA (cg0873)

This study

KC315

KC314 derivate; bΔpobA (cg1226), Ptuf_qsuC (cg0503)

This study

KC367

KC315 derivate; cPtuf_aroC (cg1829)

This study

KC376

KC367 derivate; dPtuf_tkt (cg1774)

This study

KC408

KC376 derivate; Ptuf_ppsA (cg0644)

This study

KC525

KC408 derivate; ΔqsuB (cg0502)

This study

KC551

KC525 derivate; ePtuf_pabAB (cg1134)

This study

KC594

KC551 derivate; fPtuf_aroGD146N

This study

KC617

KC594 derivate; gPtuf_aroFP155L

This study

KN001

hKmR; KC551 harboring pKCG_Ptuf_T1

This study

KN002

KmR; KC551 harboring pKCG_Ptuf_BdPHBH_T1

This study

KN003

KmR; KC551 harboring pKCG_Ptuf_CvPHBH_T1

This study

KN004

KmR; KC551 harboring pKCG_Ptuf_RpPHBH_T1

This study

KN005

KmR; KC551 harboring pKCG_Ptuf_SmPHBH_T1

This study

KN006

KmR; KC551 harboring pKCG_Ptuf_CmPHBH_T1

This study

KN007

KmR; KC551 harboring pKCG_Ptuf_RfPHBH_T1

This study

KN008

KmR; KC551 harboring pKCG_Ptuf_CvPHBHY201F_T1

This study

KN009

KmR; KC551 harboring pKCG_Ptuf_CvPHBHY201S_T1

This study

KN010

KmR; KC551 harboring pKCG_Ptuf_CvPHBHY201T_T1

This study

KN011

KmR; KC551 harboring pKCG_Ptuf_CvPHBHT294G_T1

This study

KN012

KmR; KC551 harboring pKCG_Ptuf_CvPHBHT294A_T1

This study

KN013

KmR; KC551 harboring pKCG_Ptuf_CvPHBHT294V_T1

This study

KN014

KmR; KC551 harboring pKCG_Ptuf_CvPHBHT294L_T1

This study

KN015

KmR; KC551 harboring pKCG_Ptuf_CvPHBHT294I_T1

This study

KN016

KmR; KC551 harboring pKCG_Ptuf_CvPHBHT294S_T1

This study

KN017

KmR; KC551 harboring pKCG_Ptuf_CvPHBHT294C_T1

This study

KN018

KmR; KC551 harboring pKCG_Ptuf_CvPHBHY201F/T294S_T1

This study

KN019

KmR; KC551 harboring pKCG_Ptuf_CvPHBHY161S/D357V_T1

This study

KN020

KmR; KC551 harboring pKCG_Ptuf_CvPHBHY161S_T1

This study

KN021

KmR; KC551 harboring pKCG_Ptuf_CvPHBHD357V_T1

This study

KN022

KmR; KC551 harboring pKCG_Ptuf_CvPHBHM106G_T1

This study

KN023

KmR; KC551 harboring pKCG_Ptuf_CvPHBHM106A_T1

This study

KN024

KmR; KC551 harboring pKCG_Ptuf_CvPHBHM106V_T1

This study

KN025

KmR; KC551 harboring pKCG_Ptuf_CvPHBHM106L_T1

This study

KN026

KmR; KC551 harboring pKCG_Ptuf_CvPHBHM106I_T1

This study

KN027

KmR; KC551 harboring pKCG_Ptuf_CvPHBHM106S_T1

This study

KN028

KmR; KC551 harboring pKCG_Ptuf_CvPHBHM106T_T1

This study

KN029

KmR; KC551 harboring pKCG_Ptuf_CvPHBHM106C_T1

This study

KN030

KmR; KC551 harboring pKCG_Ptuf_CvPHBHM106A/T294S_T1

This study

KN031

KmR; KC551 harboring pKCG_Ptuf_CvPHBHM106A/Y201F/T294S_T1

This study

KN032

KmR; KC617 harboring pKCG_Ptuf_CvPHBHY161S/D357V_T1

This study

KN033

KmR; KC617 harboring pKCG_Ptuf_CvPHBHM106A_T1

This study

KN034

KmR; KC617 harboring pKCG_Ptuf_CvPHBHM106A/T294S_T1

This study

KN035

KmR; KC617 harboring pKCG_Ptuf_CvPHBHM106A/Y201F/T294S_T1

This study

  1. a NBRC: Biological Resource Center, National Institute of Technology and Evaluation, Japan
  2. b The endogenous pobA (cg1226) gene encoding PHBH was disrupted to prevent potential background 3-hydroxylation
  3. c The aroK (cg1828) gene is downstream and constitutes an operon
  4. d The tal (cg1776), zwf (cg1778), opcA (cg1779), and pgl (cg1780) genes are downstream and constitute an operon
  5. e The pabC (cg1135) gene is downstream and constitutes an operon
  6. f aroGD146N: the codon-optimized aroG gene variant encoding D146N mutant of DAHP synthase from Escherichia coli
  7. g aroFP155L: the aroF (cg1129) gene variant encoding P155L mutant of DAHP synthase
  8. h KmR: kanamycin resistance