Fig. 7
In silico simulation and fed-batch culture for calculation of the carbon yield. Open and filled circles indicate 4-aminobenzoic acid (4-ABA) and 4-amino-3-hydroxybenzoic acid (4,3-AHBA) production, respectively. A The theoretical yield calculated from each individual flux balance analysis (FBA) was plotted as the O2 uptake rate was varied between 0 and 30 mmol/(gDW·h). The growth rate was fixed at 0 mmol/(gDW·h). B The theoretical yield calculated from each individual FBA was plotted as the growth rate was varied between 0 and 0.6 mmol/(gDW·h). The O2 uptake rate was fixed at 20 mmol/(gDW·h). C The fed-batch culture of KN034 expressing CvPHBHM106A/T294S in chemically defined CGXII medium