Fig. 6
Production of 4-amino-3-hydroxybenzoic acid (4,3-AHBA) in fed-batch culture with nutrient-rich CGTG15 medium using Corynebacterium glutamicum strains expressing the selected CvPHBH mutants. A, B Time course of 4,3-AHBA and 4-aminobenzoic acid (4-ABA) concentrations in culture supernatants. The color of circles and squares indicates the following strains: black, KN032 (CvPHBHY161S/D357V); green, KN033 (CvPHBHM106A); orange, KN034 (CvPHBHM106A/T294S); and blue, KN035 (CvPHBHM106A/Y201F/T294S). C, D Fed-batch culture of KN034 performed for longer duration. Each plot indicates: open square, OD600; filled square, glucose concentration; open circle, 4-ABA concentration; filled circle, 4,3-AHBA concentration. Time variations of fermentation process parameters are shown in Additional file 2: Fig. S6