Fig. 2
Screening of wild type 4-hydroxybenzoate 3-hydroxylases (PHBHs) under an adequate supply of 4-aminobenzoic acid (4-ABA) in Corynebacterium glutamicum. A Time-dependent 4-ABA production of KC551 in culture with nutrient-rich CGTG15 medium. Open and filled circles indicate the concentration of 4-ABA and glucose, respectively. Open squares indicate OD600. B The results of HPLC analysis of the culture supernatants of C. glutamicum strains expressing heterologous PHBHs. White and gray colored bars indicate the concentration of 4-ABA and 4-amino-3-hydroxybenzoic acid (4,3-AHBA), respectively. Data are represented as mean ± standard deviation of biological replicates (n = 6). Yellow circles indicate the mean percentage of 4,3-AHBA. The horizontal axis indicates the strains of interest expressing wild type PHBHs. N.D.: not detected