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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Heterologous production of the insecticidal pea seed albumin PA1 protein by Pichia pastoris and protein engineering to potentiate aphicidal activity via fusion to snowdrop lectin Galanthus nivalis agglutinin; GNA)

Fig. 4

Average pixel intensity of A. pisum guts from confocal imaging of a pulse-chase experiment with FITC labelled proteins (representative images in Fig. 3). GNA, PAF and PAF/GNA were fed at 8 µM and ovalbumin at 5 µM. Pixel intensity was measured using the measure function of FIJI, the gut was traced using the outline provided by the PI counterstain and fluorescence in the green wavelength recorded. Mean pixel intensity was recorded for a minimum of 6 guts per treatment and standard error was calculated. Pairwise t-tests were used to determine statistical significance * denotes p < 0.05 or ** p < 0.0005 when compared to equivalent PAF/GNA timepoint

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Microbial Cell Factories

ISSN: 1475-2859

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