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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Heterologous production of the insecticidal pea seed albumin PA1 protein by Pichia pastoris and protein engineering to potentiate aphicidal activity via fusion to snowdrop lectin Galanthus nivalis agglutinin; GNA)

Fig. 1

a Schematic of constructs encoding recombinant PAF and PAF/GNA produced in the yeast P. pastoris showing predicted molecular masses; tag denotes the presence of a six-residue histidine sequence enabling protein purification by nickel affinity chromatography and immunoblot detection. b Separation of purified proteins by SDS-PAGE gel (17.5% acrylamide) stained for total protein: loading of proteins in µg is depicted. GNA standards are GNA purified from snowdrop bulbs (Sigma-Aldrich, St. Louis, USA). c Western analysis of recombinant proteins using anti-His (400 ng protein loaded) and anti-GNA (200 ng protein loaded) antibodies. Location of mass markers run on the same gel are depicted

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Microbial Cell Factories

ISSN: 1475-2859

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