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Table 2 BOT-A2 promoter table with DE number

From: Expanding the genetic toolbox of Rhodotorula toruloides by identification and validation of six novel promoters induced or repressed under nitrogen starvation

Promoter name

Gene annotation

Simplified strain names

Glucose

Xylose

Countsnorm t1

Countsnorm t2

Log2 FC t2 vs t1

p-value t2 vs t1

Countsnorm t1

Countsnorm t2

Log2 FC t2 vs t1

p-value t2 vs t1

3877

Nitrate transporter

UP1a–UP1e

5.8 ± 0.6

1963.6 ± 161.6

8.4

0

3.5 ± 0.4

619.3 ± 9.9

7.7

0

4847

Alcohol oxidase Fao1p

UP2a–UP2e

32.4 ± 3.0

608.1 ± 91.7

4.2

9.5e-83

14.9 ± 3.5

281.5 ± 44.1

4.5

1.6e-89

0480

Carboxypeptidase Y inhibitor Tfs1p

UP3a–UP3e

8.0 ± 2.6

310.4 ± 14.4

5.3

2.5e-75

80.1 ± 12.8

444.4 ± 22.3

2.7

4.1e-22

2184

Protein yippee-like Moh1p

UP4a–UP4e

36.9 ± 2.8

547.3 ± 112.0

3.9

5.3e-49

70.4 ± 13.1

830.9 ± 143.4

3.8

5.0e-47

6908

Autophagy-related protein

UP5a–UP5e

281.2 ± 95.5

2580.8 ± 215.5

3.2

5.4e-25

780.5 ± 159.9

4270.3 ± 697.5

2.7

3.5e-18

2391

Alcohol dehydrogenase

UP6a–UP6e

14.9 ± 2.7

282.8 ± 27.7

4.2

1.1e-55

23.2 ± 5.6

386.8 ± 47.8

4.3

2.5e-57

6709

Fatty acid elongation protein

UP7a–UP7e

182.2 ± 78.5

1227.2 ± 83.6

2.7

4.6e-19

89.4 ± 18.1

1371.3 ± 24.1

4.2

1.4e-42

0568

Protein Orm1p

UP8a–UP8e

42.8 ± 9.6

282.1 ± 11.6

2.7

8.8e-23

65.9 ± 16.8

335.7 ± 14.3

2.6

9.8e-21

5111

Dehydrogenase/reductase

UP9a-UP9e

75.9 ± 11.3

463.6 ± 35.7

2.6

4.7e-24

14.9 ± 2.9

442.8 ± 90.8

5.1

8.7e-88

0530

Ribosome-associated molecular chaperone Ssb2p

DN1a–DN1e

297.1 ± 29.8

6.0 ± 0.8

-5.6

1.6e-57

185.9 ± 80.3

3.6 ± 1.4

-5.4

3.0e-51

3356

Diphosphomevalonate decarboxylase Mvd1p

DN2a–DN2e

25.1 ± 1.9

0.7 ± 0.2

-5.2

9.9e-50

21.2 ± 4.9

0.4 ± 0.3

-5.4

1.3e-38

5449

Epsin-3 Ent3p

DN3a–DN3e

9.5 ± 0.6

0.6 ± 0.1

-4.1

2.8e-13

7.4 ± 2.8

0.8 ± 0.7

-3.0

8.6e-8

4071

APC/C activator protein Cdc20p

DN4a–DN4e

19.3 ± 5.0

1.6 ± 0.3

-3.6

4.1e-19

17.3 ± 8.3

2.2 ± 0.3

-2.7

1.6e-11

5360

2-oxoglutarate dehydrogenase (mitoch.) Kgd1p

DN5a–DN5e

102.5 ± 18.3

9.4 ± 0.7

-3.5

1.8e-42

39.4 ± 5.0

6.0 ± 1.7

-2.5

1.3e-21

5958

Mitochondrial inner membrane protein Oxa1p

DN6a–DN6e

67.0 ± 7.5

3.9 ± 0.8

-4.1

5.5e-48

33.9 ± 6.7

1.3 ± 0.5

-4.4

5.94e-50

  1. Promoters are named after four last digits of the locus tags of the genes they precede. Countsnorm are the average normalized counts from three biological replicates, normalized using the geTMM method [117]. The promoter from MEP2 (RHTO_01680) in strain NP11 was also evaluated (strains UP10a-UP10e)