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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Expanding the genetic toolbox of Rhodotorula toruloides by identification and validation of six novel promoters induced or repressed under nitrogen starvation

Fig. 6

Fluorescence reporter analysis of strains UP3e and UP10a. UP3e (promoter 0480p, from the gene RTBOTA2_000480 putatively encoding the Carboxypeptidase Y inhibitor Tfs1p) (A) and UP10a (promoter MEP2p from NP11) (B) were cultivated in shake flasks in three different media (CN40, CN80, CN7.5). GFP was analysed using flow cytometry; 100,000 events were captured. Ammonia consumption profiles are available in Additional file 1: Figure S3 and glucose and OD620nm in Additional file 1: Figure S3. Error bars refer to standard deviation from two biological replicates

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