Fig. 6From: Expanding the genetic toolbox of Rhodotorula toruloides by identification and validation of six novel promoters induced or repressed under nitrogen starvationFluorescence reporter analysis of strains UP3e and UP10a. UP3e (promoter 0480p, from the gene RTBOTA2_000480 putatively encoding the Carboxypeptidase Y inhibitor Tfs1p) (A) and UP10a (promoter MEP2p from NP11) (B) were cultivated in shake flasks in three different media (CN40, CN80, CN7.5). GFP was analysed using flow cytometry; 100,000 events were captured. Ammonia consumption profiles are available in Additional file 1: Figure S3 and glucose and OD620nm in Additional file 1: Figure S3. Error bars refer to standard deviation from two biological replicatesBack to article page