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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Expanding the genetic toolbox of Rhodotorula toruloides by identification and validation of six novel promoters induced or repressed under nitrogen starvation

Fig. 5

Analysis of strain UP1a. UP1a (promoter 3877p, from the gene RTBOTA2_003877 encoding a putative nitrate transporter) was cultivated in shake flasks in four different media (CN40, CN80, CN160, YNB1x). GFP fluorescence intensity (A) was analysed using flow cytometry; 100,000 events were captured. Ammonia concentration (B) was analysed using an enzymatic kit. OD620nm and glucose consumption are available in Additional file 1: Figure S7, and ammonia concentration on the control medium YNB1x in Additional file 1: Figure S10. Error bars refer to standard deviation from two biological replicates

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Microbial Cell Factories

ISSN: 1475-2859

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