Fig. 2
Differential gene expression analysis during shake flask cultivations of BOT-A2 on glucose and xylose. Parameters measured include growth as OD600nm A, sugar concentration for glucose or xylose B, ammonia concentration C, and lipid concentration in mg lipids/g CDW at growth on glucose and on xylose D. All experiments were performed in biological triplicates and error bars represent the standard deviations. RNAseq sampling time points are shown in C. RNAseq samples were taken at two time points with g1 = time point 1 during exponential growth on glucose, x1 = time point 1 during exponential growth on xylose, and g2 = time point 2 during nitrogen-starvation phase on glucose, and x2 = time point 2 during nitrogen-starvation phase on xylose. Red and ■ = BOT-A2 on glucose, Yellow and ● = BOT-A2 on xylose. The Venn diagram (E) illustrates all 2095 significantly differentially expressed genes (fulfilling cut-off values of |log2 fold change|≥ 2 and adjusted p-value ≤ 10–6) identified in the four different comparisons: g2g1—differential gene expression during nitrogen starvation on glucose; x2x1—differential gene expression during nitrogen starvation on xylose; x1g1—differential gene expression during exponential growth on xylose compared to glucose; x2g2—differential gene expression during nitrogen-starvation on xylose compared to glucose