Fig. 8

CT265 EVs are cytotoxic to macrophages and are taken up by macrophages. (A and B) HD11 and PMA-activated THP-1 macrophages were incubated with different concentrations of CT265 EVs (30, 40, or 50 µg/mL) for 24 h. Cell viability was determined with a CCK-8 assay. At each time point, data were normalized to the untreated control. Data are shown as the means ± SEM of three independent experiments. Statistics: one-way ANOVA, ^*P < 0.05, ^**P < 0.01 compared with no treatment. Data are the means of three biological replicates ± SEM. (C and D) HD11 and PMA-activated THP-1 macrophages were treated with 50 µg/mL DiO-labeled EVs and incubated for 4, 8, 12, or 16 h. The cells were fixed and stained with DAPI (blue) and phalloidin (red). The figure represents one of three independent experiments