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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Extracellular vesicles produced by avian pathogenic Escherichia coli (APEC) activate macrophage proinflammatory response and neutrophil extracellular trap (NET) formation through TLR4 signaling

Fig. 3

EVs induced an inflammatory response in macrophages. (A) Expression levels of inflammasome-related proteins in PMA-treated THP-1 macrophages, pretreated or not pretreated with TAK242 and incubated for 12 h with EVs (30, 40, or 50 µg/mL), were measured with a western blotting assay. A representative western blot is shown. (B) Densitometric analysis was used to quantify the levels of inflammasome-related proteins. Statistical test: two-way ANOVA, *P < 0.05, **P < 0.01. (C + D + E) PMA-treated THP-1 macrophages, pretreated or not pretreated with TAK242, were incubated for 12 h with EVs (30, 40, or 50 µg/mL), and the expression levels of IL1β, IL18, and TNF-α were determined with ELISAs. Statistical test: one-way ANOVA, *P < 0.05, **P < 0.01

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Microbial Cell Factories

ISSN: 1475-2859

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