Cost-effective production of alginate oligosaccharides from Laminaria japonica roots by Pseudoalteromonas agarivorans A3

Table 4 Experiment design of central composite design (CCD) experiment and corresponding response

Run	X₁		X₂		X₃		X₄		Enzyme activity (U/ml)
Run	Coded level	Real level (%)	Coded level	Real level (^oC)	Coded level	Real level	Coded level	Real level (%)	Enzyme activity (U/ml)
1	− 1	1.75	− 1	18.5	− 1	7.85	− 1	1.5	6.53
2	1	2.25	− 1	18.5	− 1	7.85	− 1	1.5	7.40
3	− 1	1.75	1	20.5	− 1	7.85	− 1	1.5	5.08
4	1	2.25	1	20.5	− 1	7.85	− 1	1.5	6.23
5	− 1	1.75	− 1	18.5	1	8.15	− 1	1.5	7.04
6	1	2.25	− 1	18.5	1	8.15	− 1	1.5	7.44
7	− 1	1.75	1	20.5	1	8.15	− 1	1.5	6.1
8	1	2.25	1	20.5	1	8.15	− 1	1.5	6.93
9	− 1	1.75	− 1	18.5	− 1	7.85	1	2	6.86
10	1	2.25	− 1	18.5	− 1	7.85	1	2	7.26
11	− 1	1.75	1	20.5	− 1	7.85	1	2	6.75
12	1	2.25	1	20.5	− 1	7.85	1	2	6.85
13	− 1	1.75	− 1	18.5	1	7.85	1	2	7.48
14	1	2.25	− 1	18.5	1	7.85	1	2	7.64
15	− 1	1.75	1	20.5	1	7.85	1	2	7.47
16	1	2.25	1	20.5	1	7.85	1	2	8.01
17	− 2	1.5	0	19.5	0	8	0	1.75	5.69
18	2	2.5	0	19.5	0	8	0	1.75	6.35
19	0	2	− 2	17.5	0	8	0	1.75	7.46
20	0	2	2	21.5	0	8	0	1.75	6.49
21	0	2	0	19.5	− 2	7.7	0	1.75	5.34
22	0	2	0	19.5	2	8.3	0	1.75	6.21
23	0	2	0	19.5	0	8	− 2	1.25	6.80
24	0	2	0	19.5	0	8	2	2.25	8.60
26	0	2	0	19.5	0	8	0	1.75	8.52
26	0	2	0	19.5	0	8	0	1.75	8.34
27	0	2	0	19.5	0	8	0	1.75	8.24
28	0	2	0	19.5	0	8	0	1.75	7.91
29	0	2	0	19.5	0	8	0	1.75	7.94
30	0	2	0	19.5	0	8	0	1.75	8.31

X₁, NH₄NO₃ (w/v, %); X₂, fermentation temperature (°C); X₃, pH; X₄, inoculum size (%)

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